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:: Volume 11, Issue 1 (1-2024) ::
2024, 11(1): 68-78 Back to browse issues page
The Impact of Aerobic Exercise and Psilocybin on Methamphetamine-Induced Histopathological Changes in Rat Cerebral Cortex
Fariba Rasannezhad , Asieh Abbassi-Daloii , Seyyed Javad Ziaolhagh , Ahmad Abdi
Department of Sport physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran , abbasi.daloii@gmail.com
Abstract:   (184 Views)
Introduction: Methamphetamine addiction is one of the most prevalent substance use disorders. Augmenting traditional drug treatments with non-pharmacological interventions can enhance treatment efficacy and sustainability. This study aims to investigate the impact of aerobic exercise and psilocybin on histopathological changes in the cerebral cortex of methamphetamine-induced rats.
Material & Methods: This experimental research involved 30 female rats randomly allocated into five groups: control, methamphetamine, methamphetamine + aerobic exercise, methamphetamine + psilocybin, and methamphetamine + psilocybin + aerobic exercise. Rats received 15 mg of methamphetamine intraperitoneally every 12 hours for four days. Aerobic exercise was conducted on a treadmill with an 8-week program of escalating intensity, reaching a maximum running time of 30 minutes and speed of 25 m/min, with a 5% slope introduced in the fourth week. Psilocybin was administered intraperitoneally at a microdose of 0.025 mg/kg over 24 sessions. Histopathological changes were assessed using hematoxylin and eosin staining, and descriptive analysis with images was employed to interpret cortical brain tissue samples.
Results: Histological examination revealed tissue and cellular disorganization, altered morphology of pyramidal neurons, and reduced microglial cell counts in methamphetamine-induced rats compared to controls. Endurance training, psilocybin supplementation, and their combination exhibited improvements in tissue and cellular alterations in the cerebral cortex of addicted rats.
Conclusion:  This study demonstrates that exercise training and psilocybin administration in methamphetamine-exposed rats ameliorate cortical histopathology, with the combined intervention yielding the most significant effects. This synergistic effect may reduce the risk of relapse in methamphetamine users by positively modulating brain and central nervous system functions.
 
Keywords: Methamphetamine, Aerobic Exercise, Psilocybin, cerebral Cortex, Rats
Full-Text [PDF 1075 kb]   (67 Downloads)    
Type of Study: Research | Subject: Physiology
Received: 2023/04/29 | Accepted: 2023/05/8 | Published: 2024/01/20
References
1. Benthin A, Slovic P, Severson H. A psychometric study of adolescent risk perception. J Adolesc. 1993; 16(2):153-68.
2. Krasnova IN, Cadet JL. Methamphetamine toxicity and messengers of death. Brain Res Rev.2009; 60(2):379-407.
3. Fleckenstein AE, Volz TJ, Riddle EL, Gibb JW, Hanson GR. New insights into the mechanism of action of amphetamines. Annu. Rev. Pharmacol. Toxicol. 2007; 47:681-98.
4. Barr AM, Panenka WJ, MacEwan GW, Thornton AE, Lang DJ, Honer WG and et al. The need for speed: an update on methamphetamine addiction. J Psychiatr Neurosci. 2006; 31(5):301-13. https://www.jpn.ca/content/31/5/301.short
5. Baptista S, Lasgi C, Benstaali C, Milhazes N, Borges F, Fontes-Ribeiro C and et al. Methamphetamine decreases dentate gyrus stem cell self-renewal and shifts the differentiation towards neuronal fate. Stem Cell Res. 2014; 13(2):329-41.
6. Mokri A. Brief overview of the status of drug abuse in Iran. 2002. file:///C:/Users/u/Downloads/86920020312%20(3).
7. Huang Z, Wei X, Wang Y, Tian J, Dong J, Liang B and et al. Psilocybin Promotes Cell-Type-Specific Changes in the Orbitofrontal Cortex Revealed by Single-Nucleus RNA-seq. bioRxiv. 2024; 2024-01.
8. Nkadimeng SM, Nabatanzi A, Steinmann CM, Eloff JN. Phytochemical, cytotoxicity, antioxidant and anti-inflammatory effects of Psilocybe natalensis magic mushroom. Plants. 2020; 9(9):1127.
9. Zanikov T, Gerasymchuk M, Ghasemi Gojani E, Robinson GI, Asghari S, Groves A, Haselhorst L, Nandakumar S, Stahl C, Cameron M, Li D. The Effect of Combined Treatment of Psilocybin and Eugenol on Lipopolysaccharide-Induced Brain Inflammation in Mice. Molecules. 2023 Mar 14;28(6):2624.
10. Nkadimeng SM, Hay L, Steinmann CM, Eloff JN. Administration effects of four psilocybin mushroom extracts on serotonin levels and endothelial nitric oxide synthase activity levels in vivo and in vitro after one hour. J Inflamm Res.
11. Hibicke M, Landry AN, Kramer HM, Talman ZK, Nichols CD. Psychedelics, but not ketamine, produce persistent antidepressant-like effects in a rodent experimental system for the study of depression. ACS Chem Neurosci. 2020; 11(6):864-71.
12. Gotvaldová K, Hájková K, Borovička J, Jurok R, Cihlářová P, Kuchař M. Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cubensis. Drug Test Anal. 2021; 13(2):439-46.
13. Nkadimeng SM, Steinmann CM, Eloff JN. Effects and safety of Psilocybe cubensis and Panaeolus cyanescens magic mushroom extracts on endothelin-1-induced hypertrophy and cell injury in cardiomyocytes. Sci Rep. 2020; 10(1):1-1. https://www.nature.com/articles/s41598-020-79328-5
14. Rosa HZ, Barcelos RC, Segat HJ, Roversi K, Dias VT, Milanesi LH, Burger ME. Physical exercise modifies behavioral and molecular parameters related to opioid addiction regardless of training time. Eur Neuropsychopharmacol. 2020 Mar 1;32:25-35.
15. He Q, Wu J, Wang X, Luo F, Yan K, Yu W, Mo Z, Jiang X. Exercise intervention can reduce the degree of drug dependence of patients with amphetamines/addiction by improving dopamine level and immunity and reducing negative emotions. Am J Transl Res. 2021;13(3):1779. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8014419/.
16. Bahreini Pour MA. Investigation the effect of low-intensity aerobic training for 10 weeks along with blood flow restriction on amount of protein BDNF in soleus and EDL muscles as well as the sciatic nerve in aged male rats. J Sport Exerc Physiol. 2019; 12(1):59-75.
17. Mahalakshmi B, Maurya N, Lee SD, Bharath Kumar V. Possible neuroprotective mechanisms of physical exercise in neurodegeneration. Int J Mol Sci. 2020 Aug 16;21(16):5895.
18. Farì G, Lunetti P, Pignatelli G, Raele MV, Cera A, Mintrone G, Ranieri M, Megna M, Capobianco L. The effect of physical exercise on cognitive impairment in neurodegenerative disease: from pathophysiology to clinical and rehabilitative aspects. Int J Mol Sci. 2021 Oct 27;22(21):11632.
19. Zhang KK, Wang H, Qu D, Chen LJ, Wang LB, Li JH and et al. Luteolin alleviates methamphetamine-induced hepatotoxicity by suppressing the p53 pathway-mediated apoptosis, autophagy, and inflammation in rats. Front Pharmacol. 2021; 12:641917.
20. Marques E, Vasconcelos F, Rolo MR, Pereira FC, Silva AP, Macedo TR and et al. Influence of Chronic Exercise on the Amphetamine‐Induced Dopamine Release and Neurodegeneration in the Striatum of the Rat. Ann N Y Acad Sci. 2008; 1139(1):222-31.
21. Vieu T, Morais AP, Pasechnik R. Electroweak phase transitions in multi-Higgs models: the case of trinification-inspired THDSM. J Cosmol Astropart Phys. 2018; 2018(07):014. DOI: 10.1088/1475-7516/2018/07/014
22. Alkadhi KA, Dao AT. Exercise decreases BACE and APP levels in the hippocampus of a rat model of Alzheimer's disease. Mol Cell Neurosci. 2018; 86:25-9.
23. Brené S, Bjørnebekk A, Åberg E, Mathé AA, Olson L, Werme M. Running is rewarding and antidepressive. Physiol Behav. 2007; 92(1-2):136-40.
24. Janse Van Rensburg K, Taylor A, Hodgson T, Benattayallah A. Acute exercise modulates cigarette cravings and brain activation in response to smoking-related images: an fMRI study. Psychopharmacology. 2009; 203:589-98. https://link.springer.com/article/10.1007/s00213-008-1405-3
25. Wang D, Zhu T, Zhou C, Chang YK. Aerobic exercise training ameliorates craving and inhibitory control in methamphetamine dependencies: a randomized controlled trial and event-related potential study. Psychol Sport Exerc. 2017; 30:82-90.
26. Park M, Levine H, Toborek M. Exercise protects against methamphetamine-induced aberrant neurogenesis. Sci Rep. 2016; 6(1):34111. https://www.nature.com/articles/srep34111
27. Radak Z, Boldogh I. 8-Oxo-7, 8-dihydroguanine: links to gene expression, aging, and defense against oxidative stress. Free Radic Biol Med. 2010; 49(4):587-96.
28. Liśkiewicz A, Przybyła M, Park M, Liśkiewicz D, Nowacka-Chmielewska M, Małecki A and et al. Methamphetamine-associated cognitive decline is attenuated by neutralizing IL-1 signaling. Brain Behav Immun.2019; 80:247-54.
29. Speck AE, Tromm CB, Pozzi BG, Paganini CS, Tuon T, Silveira PC and et al. The dose-dependent antioxidant effects of physical exercise in the hippocampus of mice. Neurochem Res. 2014; 39:1496-501. https://link.springer.com/article/10.1007/s11064-014-1339-6
30. Toborek M, Seelbach MJ, Rashid CS, András IE, Chen L, Park M and et al. Voluntary exercise protects against methamphetamine-induced oxidative stress in brain microvasculature and disruption of the blood–brain barrier. Mol Neurodegener. 2013; 8:1-1. https://link.springer.com/article/10.1186/1750-1326-8-22
31. Coppola M, Tirrito E, Karevski D, Collura M. Growth of entanglement entropy under local projective measurements. Phys Rev. 2022; 105(9):094303. https://journals.aps.org/prb/abstract/10.1103/PhysRevB.105.094303
32. Bogenschutz MP, Forcehimes AA, Pommy JA, Wilcox CE, Barbosa PC, Strassman RJ. Psilocybin-assisted treatment for alcohol dependence: a proof-of-concept study. J Psychopharmacol. 2015; 29(3):289-99.
33. Du Jardin KG, Liebenberg N, Cajina M, Müller HK, Elfving B, Sanchez C and et al. S-ketamine mediates its acute and sustained antidepressant-like activity through a 5-HT1B receptor dependent mechanism in a genetic rat model of depression. Front Pharmacol. 2018; 8:978.
34. Daniel J, Haberman M. Clinical potential of psilocybin as a treatment for mental health conditions. Ment Health Clin. 2017; 7(1):24-8.
35. Kraehenmann R, Preller KH, Scheidegger M, Pokorny T, Bosch OG, Seifritz E and et al. Psilocybin-induced decrease in amygdala reactivity correlates with enhanced positive mood in healthy volunteers. Biol Psychiatry. 2015; 78(8):572-81.
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Rasannezhad F, Abbassi-Daloii A, Ziaolhagh S J, Abdi A. The Impact of Aerobic Exercise and Psilocybin on Methamphetamine-Induced Histopathological Changes in Rat Cerebral Cortex. Journal of Basic Research in Medical Sciences 2024; 11 (1) :68-78
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Volume 11, Issue 1 (1-2024) Back to browse issues page
مجله ی تحقیقات پایه در علوم پزشکی Journal of Basic Research in Medical Sciences
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