Journal of Basic Research in Medical Sciences
J Basic Res Med Sci
Volume 12, Issue 4 (9-2025)
jbrms 2025, 12(4): 64-76 |
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Nikpour H, Seidkhani-Nahal A, Golshani S, Basati G. Evaluation of Serum Inosine, Hypoxanthine, and Carnitine as Ischemia Markers of Heart Disease. jbrms 2025; 12 (4) :64-76
URL: http://jbrms.medilam.ac.ir/article-1-964-en.html
URL: http://jbrms.medilam.ac.ir/article-1-964-en.html
Department of Clinical Biochemistry, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran , gholam.basati@gmail.com
Abstract: (23 Views)
Introduction: Heart disease (HD) is a major global death cause, with adenosine triphosphate metabolites, carnitine, and other biomarkers aiding in timely detection and disease prediction. This study aims to investigate the changes in inosine, hypoxanthine, and carnitine levels in the serum of ischemic HD patients and to examine the relationship of these components with the extent of coronary artery blockage, as well as for the detection of coronary artery disease (CAD).
Materials & Methods: In this cross-sectional and case-control study, from patients who underwent angiographic assessment for suspected CAD, 40 control subjects, 40 stable angina patients, 40 unstable angina patients, and 40 myocardial infarction patients were selected. Serum samples were collected, and the amounts of inosine, hypoxanthine, and carnitine were measured using an HPLC-UV-visible method. The differences of the three substances in the patients and their clinical importances were analyzed by SPSS V.22 (P < 0.05).
Results: The results of this study showed that the levels of inosine and hypoxanthine in patients were higher than those in the control group (P < 0.01), while the level of carnitine was lower than that in the control group (P = 0.04). Conversely, the concentration of carnitine in patients was lower than in the healthy control group, with respective concentrations of 31.84 (28.58-35.62), 34.01 (28.25-37.13), and 33.59 (28.58-38.87) µg/ml.
Conclusion: This study indicated that inosine and hypoxanthine could be effective and rapid discriminators, in comparison with other heart biomarkers, for the timely detection of HD.
Materials & Methods: In this cross-sectional and case-control study, from patients who underwent angiographic assessment for suspected CAD, 40 control subjects, 40 stable angina patients, 40 unstable angina patients, and 40 myocardial infarction patients were selected. Serum samples were collected, and the amounts of inosine, hypoxanthine, and carnitine were measured using an HPLC-UV-visible method. The differences of the three substances in the patients and their clinical importances were analyzed by SPSS V.22 (P < 0.05).
Results: The results of this study showed that the levels of inosine and hypoxanthine in patients were higher than those in the control group (P < 0.01), while the level of carnitine was lower than that in the control group (P = 0.04). Conversely, the concentration of carnitine in patients was lower than in the healthy control group, with respective concentrations of 31.84 (28.58-35.62), 34.01 (28.25-37.13), and 33.59 (28.58-38.87) µg/ml.
Conclusion: This study indicated that inosine and hypoxanthine could be effective and rapid discriminators, in comparison with other heart biomarkers, for the timely detection of HD.
Type of Study: Research |
Subject:
Clinical Biochemistry
Received: 2025/04/12 | Accepted: 2025/06/6 | Published: 2025/10/5
Received: 2025/04/12 | Accepted: 2025/06/6 | Published: 2025/10/5
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