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E-test antibiotic susceptibility of E.coli strains isolated from hospital acquired infections of Imam Khomeini hospital, Ilam, Iran
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Lida Bimanand   , Norkhoda Sadeghifard , Farid Azizi Jalilian , Hassan Hossainzadegan , Sattar Mohammadi Darbidi , Ali Hemahian , Sobhan Ghafourian , Iraj Pakzad |
| Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam , pakzadi2006@gmail.com |
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Abstract: (4497 Views) |
Introduction: Escherichia coli (E.coli) as a main cause of both nosocomial and community-acquired infections in humans have a relative potential to develop resistance. Nowadays, most infections caused by ESBL-producing E.coli (ESBLEC) had mostly been described as nosocomial acquired or nursing home related. In this study, we employed E-test assay to detect antibiotic resistance of E.coli strains and determine MIC of antibiotics.
Materials and methods: Thirty E.coli strains gathered from Imam Khomeini hospital of Ilam, and cultured on TSB and bacterial suspension prepared by 0.5 µF concentration for E-test. Mueller Hinton agar and E-test strips of Amikacin, Cefepime, Ceftazidime, Ceftriaxone, Gentamicin, Meropenem, Nitrofurantoin, Piperacillin/Tazobactam, Tetracycline, Ticarcillin/ Clavulanic acid, Tobramycin, Trimethoprim were used
Results: Resistance to Ceftriaxone, Tobramycin, Gentamicin, Ticarcillin/ Clavulanic, Amikacin were 19.8%, 26.4%, 23.3%, 62.7%, 70.3%, respectively.
Conclusion: the results indicated, E.coli strains in this study were high sensitivity to Meropenem ,Nitrofuratoin, Ciprofloxacin , Ceftazidime, Cefepime. |
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| Keywords: E-test, Antibiotic susceptibility, E.coli, Ilam, Iran |
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Full-Text [PDF 697 kb]
(1300 Downloads)
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Type of Study: Research |
Subject:
Clinical microbiology
Received: 2015/07/5 | Accepted: 2015/10/5 | Published: 2016/07/14
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