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:: Volume 4, Issue 2 (3-2017) ::
JBRMS 2017, 4(2): 50-57 Back to browse issues page
Mesenchymal stem cells as a reference cell for HLA-typing
Roohollah Mirzaee Khalilabadi, Fatemeh Yari, Naser Amirizadeh, Mahin Nikougoftar, Arezoo Oodi
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran , f.yari@ibto.ir
Abstract:   (3646 Views)

Introduction: Recognition of human leukocyte antigens (HLA) is of importance for hematopoietic stem cell transplantation. Any HLA-mismatches between the donor and recipient can cause graft rejection or other complications. In HLA-typing experiments, usage of HLA-known reference cells accompany with HLA-unknown samples is obligatory. Some international centers represent these cells with high expenses. On the other hand, transferring of these cells is problematic and in some instances is not practical.  In this study, we introduced umbilical cord-derived mesenchymal stem cells (MSCs) as reference cells for HLA genotyping. These cells are national and can be prepared locally.

Materials and methods: We isolated MSCs from three umbilical cord and after their growth and proliferation, these cells were characterized by flow cytometry technique using antibodies to CD29, CD34, CD44, CD45, CD73, CD90 and CD105. HLA-typing was then carried out by PCR-SSP kits for HLA-A, -B and -DRB allele’s identification.

Results: Isolated MSCs were positive for MSCs markers; CD29, CD44, CD73, CD90, and CD105 and negative for hematopoietic stem cell markers; CD34 and CD45. HLA alleles were determined. One of the samples was homologous for HLA alleles and the others were heterologous.

Conclusion: We can develop a reference panel for HLA-typing by obtaining MSCs from available sources like umbilical cord.

Keywords: HLA-typing, Mesenchymal stem cells, Reference panel, Umbilical cord
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Type of Study: Research | Subject: Immunology
Received: 2016/01/2 | Accepted: 2016/07/1 | Published: 2016/09/30
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Mirzaee Khalilabadi R, Yari F, Amirizadeh N, Nikougoftar M, Oodi A. Mesenchymal stem cells as a reference cell for HLA-typing . JBRMS 2017; 4 (2) :50-57
URL: http://jbrms.medilam.ac.ir/article-1-227-en.html

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Volume 4, Issue 2 (3-2017) Back to browse issues page
مجله ی تحقیقات پایه در علوم پزشکی Journal of Basic Research in Medical Sciences
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