[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
About Journal::
Editorial Board::
Articles Archive::
Indexing Databases::
To Authors::
To Reviewers::
Registration::
Submit Your Article::
Policies and Publication Ethics::
Archiving Policy::
Site Facilities::
Contact Us::
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations740600
h-index1110
i10-index1513
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE

..
:: Volume 4, Issue 2 (3-2017) ::
2017, 4(2): 50-57 Back to browse issues page
Mesenchymal stem cells as a reference cell for HLA-typing
Roohollah Mirzaee Khalilabadi , Fatemeh Yari , Naser Amirizadeh , Mahin Nikougoftar , Arezoo Oodi
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran , f.yari@ibto.ir
Abstract:   (4326 Views)

Introduction: Recognition of human leukocyte antigens (HLA) is of importance for hematopoietic stem cell transplantation. Any HLA-mismatches between the donor and recipient can cause graft rejection or other complications. In HLA-typing experiments, usage of HLA-known reference cells accompany with HLA-unknown samples is obligatory. Some international centers represent these cells with high expenses. On the other hand, transferring of these cells is problematic and in some instances is not practical.  In this study, we introduced umbilical cord-derived mesenchymal stem cells (MSCs) as reference cells for HLA genotyping. These cells are national and can be prepared locally.

Materials and methods: We isolated MSCs from three umbilical cord and after their growth and proliferation, these cells were characterized by flow cytometry technique using antibodies to CD29, CD34, CD44, CD45, CD73, CD90 and CD105. HLA-typing was then carried out by PCR-SSP kits for HLA-A, -B and -DRB allele’s identification.

Results: Isolated MSCs were positive for MSCs markers; CD29, CD44, CD73, CD90, and CD105 and negative for hematopoietic stem cell markers; CD34 and CD45. HLA alleles were determined. One of the samples was homologous for HLA alleles and the others were heterologous.

Conclusion: We can develop a reference panel for HLA-typing by obtaining MSCs from available sources like umbilical cord.

Keywords: HLA-typing, Mesenchymal stem cells, Reference panel, Umbilical cord
Full-Text [PDF 936 kb]   (2017 Downloads)    
Type of Study: Research | Subject: Immunology
Received: 2016/01/2 | Accepted: 2016/07/1 | Published: 2016/09/30
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mirzaee Khalilabadi R, Yari F, Amirizadeh N, Nikougoftar M, Oodi A. Mesenchymal stem cells as a reference cell for HLA-typing . Journal of Basic Research in Medical Sciences 2017; 4 (2) :50-57
URL: http://jbrms.medilam.ac.ir/article-1-227-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 4, Issue 2 (3-2017) Back to browse issues page
مجله ی تحقیقات پایه در علوم پزشکی Journal of Basic Research in Medical Sciences
Persian site map - English site map - Created in 0.16 seconds with 41 queries by YEKTAWEB 4653