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:: Volume 10, Issue 4 (12-2023) ::
2023, 10(4): 43-53 Back to browse issues page
Influence of Exercise Intensity on the Expression of Angiogenesis-Related Genes in the Hearts of Male Rats
Shokoufeh Kheradmand , Mohammad Reza Asad , , Reza Mir Javadi , Narges Kheradmand , Mohamad Fashi
Department of Exercise Physiology, Faculty of Physical Education and Health Sciences, Shahid- Beheshti University, Tehran, Iran , m_fashi@sbu.ac.ir
Abstract:   (287 Views)
Introduction: Angiogenesis, the formation of new capillaries from pre-existing vessels, crucially involves activation of the hypoxia-inducible factor 1 (HIF-1) and vascular endothelial growth factor (VEGF) genes. This study investigates the impact of exercise intensity on the expression of angiogenic genes in the hearts of male rats.
Material & Methods: Eighteen male Wistar rats were randomly assigned to three groups: High-Intensity Interval Training (HIIT), Continuous Training (CT), and control (C). Both HIIT and CT groups underwent 8 weeks of training with five sessions per week. Anesthesia and blood sampling occurred 48 hours post final training session. Gene levels of HIF-1 and VEGF were measured in the left ventricle. Data analysis employed ANOVA and LSD post hoc tests (P≤0.05).
Results: VEGF gene expression significantly increased in both HIIT and CT groups compared to the control group (P = 0.001), with a more pronounced elevation in the HIIT group than the CT group (P = 0.004). Furthermore, HIF-1 levels exhibited a significant reduction in both HIIT (P = 0.001) and CT (P = 0.001) groups compared to the control group, with the HIIT-induced decrease surpassing that of the CT group (P = 0.049).
Conclusion: The noteworthy elevation in VEGF and decrease in HIF-1 gene expression levels in trained rats imply that exercise training enhances angiogenesis. Importantly, the extent of this enhancement is contingent upon exercise intensity, with HIIT demonstrating more pronounced positive effects on VEGF levels.
Keywords: Exercise Intensity, Continuous Training, High-Intensity Interval Training, Heart Tissue
Full-Text [PDF 3198 kb]   (58 Downloads)    
Type of Study: Research | Subject: Physical medicine
Received: 2020/05/6 | Accepted: 2020/05/13 | Published: 2023/12/28
References
1. Kim J-R, Oberman A, Fletcher GF, Lee JY. Effect Of Exercise Intensity And Frequency On Lipid Levels In Men With Coronary Heart Disease: Training Level Comparison Trial. Am J Cardiol. 2001;87(8):942-6. doi: 10.1016/S0002-9149(01)01425-4.
2. Sylviana N, Helja N, Qolbi HH, Goenawan H, Lesmana R, Syamsunarno MRA, et al. Effect Of Swimming Exercise To Cardiac PGC-1α And HIF-1α Gene Expression In Mice. Asian J Sports Med. 2018;9(4). doi: 10.5812/Asjsm.65079.
3. Flora R, Zulkarnain M, Sorena E, Deva I, Widowati W. Correlation Between Hypoxia Inducible Factor-1α And Vesicular Endothelial Growth Factor In Male Wistar Rat Brain Tissue After Anaerobic Exercise. Trends Med Res. 2016;11(1):35-41. doi: 10.3923/Tmr.2016.35.41.
4. Nazari A, Chehelcheraghi F. Using Apelin And Exercise To Protect The Cardiac Cells: Synergic Effect In Ischemia Reperfusion Injuries Treatment In Rats. Bratisl Lek Listy. 2020;121(1):14-21. doi: 10.4149/Bll_2020_003.
5. Nourshahi M, Ranjbar K. The Stimulus Of Angiogenesis During Exercise And Physical Activity. Q Horizon Med Sci. 2013:286-96.
6. Seo DY, Kwak H, Kim AH, et al. Cardiac Adaptation To Exercise Training In Health And Disease. Pflugers Arch. 2020; 472, 155–168. doi: 10.1007/S00424-019-02266-3.
7. Ranjbar K, Nourshahi M, Hedayati M, Taheri CH. Effect Of Gender And Physical Activity On Serum Vascular Endothelial Growth Factor At Rest And Response To Submaximal Exercise. 2011;13(3):294-300.
8. Zadhoush F. Physiological Role Of Adenosine And Its Receptors In Tissue Hypoxia-Induced. Physiol Pharmacol. 2012;16(3):209-21 [In Persian].
9. Lemus-Varela M, Flores-Soto M, Cervantes-Munguia R, Torres-Mendoza B, Gudiño-Cabrera G, Chaparro-Huerta V, et al. Expression Of HIF-1α, VEGF And EPO In Peripheral Blood From Patients With Two Cardiac Abnormalities Associated With Hypoxia. Clin Biochem. 2010;43(3):234-9. doi: 10.1016/J.Clinbiochem.2009.09.022.
10. Icli B, Wara A, Moslehi J, Sun X, Plovie E, Cahill M, et al. Microrna-26a Regulates Pathological And Physiological Angiogenesis By Targeting BMP/SMAD1 Signaling. Circ Res. 2013;113(11):1231-41. doi: 10.1161/CIRCRESAHA.113.301780.
11. Ravasi A, Yadegari M, Choobineh S. Comparison Of Two Types Of Physical Activity On Response Serum VEGF-A, Non-Athletic Men. J Sport Biosci. 2014;6(1):41-56 [In Persian].
12. Hassan AF, Kamal MM. Effect Of Exercise Training And Anabolic Androgenic Steroids On Hemodynamics, Glycogen Content, Angiogenesis And Apoptosis Of Cardiac Muscle In Adult Male Rats. Int J Health Sci. 2013;7(1):47. doi: 10.12816/0006020.
13. 13. Nourshahi M, Taheri Chadorneshin H, Pirouz M. Effect Of Endurance Training In Hypoxia-Normobaric And Normal Conditions On Serum VEGF Concentration, Hemoglobin And Blood Hematocrit. Horizon Med Sci. 2012;18(3):135-40, [In Persian].
14. Kordi MR, Nekouei A, Shafiee A, Hadidi V. The Effect Of Eight Weeks High-Intensity Aerobic Continuous And Interval Training On Gene Expression Of Vascular Endothelial Growth Factor In Soleus Muscle Of Healthy Male Rats. Arak Med Univ J. 2015;18(8):53-62, [In Persian].
15. Mounier R, Pialoux V, Roels B, Thomas C, Millet G, Mercier J, Et Al. Effect Of Intermittent Hypoxic Training On HIF Gene Expression In Human Skeletal Muscle And Leukocytes. Eur J Appl Physiol. 2009;105(4):515. doi: 10.1007/S00421-008-0928-Y.
16. Lindholm ME, Fischer H, Poellinger L, Johnson RS, Gustafsson T, Sundberg CJ, Et Al. Negative Regulation Of HIF In Skeletal Muscle Of Elite Endurance Athletes: A Tentative Mechanism Promoting Oxidative Metabolism. Am J Physiol Regul Integr Comp Physiol. 2014;307(3):R248-R55. doi: 10.1152/Ajpregu.00036.2013.
17. Marschner RA, Banda P, Wajner SM, Markoski MM, Schaun M, Lehnen AM. Short-Term Exercise Training Improves Cardiac Function Associated To A Better Antioxidant Response And Lower Type 3 Iodothyronine Deiodinase Activity After Myocardial Infarction. PLoS One. 2019;14(9). doi: 10.1371/Journal.Pone.0222334.
18. Gliemann, L. Training For Skeletal Muscle Capillarization: A Janus-Faced Role Of Exercise Intensity?. Eur J Appl Physiol. 2016; 1443–1444. doi: 10.1007/S00421-016-3419-6.
19. Oka T, Akazawa H, Naito AT, Komuro I. Angiogenesis And Cardiac Hypertrophy: Maintenance Of Cardiac Function And Causative Roles In Heart Failure. Circ Res. 2014;114(3):565-71. doi: 10.1161/CIRCRESAHA.114.300507.
20. Erekat NS, Al-Jarrah MD, Al Khatib AJ. Treadmill Exercise Training Improves Vascular Endothelial Growth Factor Expression In The Cardiac Muscle Of Type I Diabetic Rats. Cardiol Res. 2014;5(1):23. doi: 10.14740/Cr314w.
21. Soori R, Amini AA, Choobineh S, Eskandari A, Behjat A, Ghram A, Et Al. Exercise Attenuates Myocardial Fibrosis And Increases Angiogenesis-Related Molecules In The Myocardium Of Aged Rats. Arch Physiol Biochem. 2019:1-6. doi: 10.1080/13813455.2019.1660370.
22. Ramezani A, Mehrialvar Y, Gaiini AA, Golab F, Kheratmand R. Effect Of A Period Of High-Intensity Interval Training On Regulation Signaling Of Factors Involved In Vascular Changes (Molecular And Tissue) Following Myocardial Ischemia. SSU_Journals. 2018;26(2):151-63, [In Persian].
23. Nourshahi M, Rostami ME, Khodagholi F. Effect Of Eight Weeks Sprint Interval Training On VEGF Rate In Aged Rats Skeletal Muscle Tissue. Majallah-I Pizishki-I Danishgah-I Ulum-I Pizishki Va Khadamat-I Bihdashti-I Darmani-I Tabriz. 2018;40(2):87-94, [In Persian].
24. Żebrowska A, Jastrzębski D, Sadowska-Krępa E, Sikora M, Di Giulio C. Comparison Of The Effectiveness Of High-Intensity Interval Training In Hypoxia And Normoxia In Healthy Male Volunteers: A Pilot Study. Biomed Res Int. 2019;2019. doi: 10.1155/2019/7315714.
25. Ghahramani M, Karbalaeifar S. The Effect Of Interval Training On Cardiac Angiogenesis Capacity In Rats With Myocardial Infarction. Rep Health Care. 2019;5(1):9-16.
26. Hoier B, Passos M, Bangsbo J, Hellsten Y. Intense Intermittent Exercise Provides Weak Stimulus For Vascular Endothelial Growth Factor Secretion And Capillary Growth In Skeletal Muscle. Exp Physiol. 2013;98(2):585-97. doi: 10.1113/Expphysiol.2012.067967.
27. Yazdanian N, Asad MR, Rahimi M. The Effect Of High Intensity Interval Training And Moderate-Intensity Continuous Training On PGC1α And VEGF In Heart Muscle Of Male Wistar Rats. Sport Physiol. 2018;10(38):111-24. doi: 10.22089/SPJ.2018.1160.
28. Shekarchizadeh P, Khazaei M, Gharakhanlou R, Karimian J, A S. The Effect Of Resistance Training On Plasma Nitric Oxide Levels, Vascular Endothelial Growth Factor And Its Type One Receptor In Healthy Male Rats. Isfahan Med Sch J. 2011;30(176).
29. Torabimehr F, Kazemi N, Hosseini SA. Effects Of Resistance And Endurance Training On HIF-1α And VEGF In Heart Tissues Of Pregnant Rats With Cadmium Toxicity. Gene, Cell And Tissue. 2019;6(1). doi: 10.5812/Gct.88363.
30. Vali Zadeh S, Motamedi P, Karami H, Rajabi H. The Effects Of Endurance Training On Gene Expression Of VEGF And VEGFR2 Of Cardiac Tissue In Type 2 Diabetic Male Wistar. J Arak Univ Med Sci. 2018;21(6):107-18, [In Persian].
31. Hadi H, Gaeini Aa, Motamedi P, Rajabi H. The Effect Of Aerobic Training On Cardiac Expression Of Mir-126 In Diabetic And Healthy Rats. 2016; 5(1), 69-78.
32. Leosco D, Rengo G, Iaccarino G, Golino L, Marchese M, Fortunato F, Et Al. Exercise Promotes Angiogenesis And Improves Β-Adrenergic Receptor Signalling In The Post-Ischaemic Failing Rat Heart. Cardiovasc Res. 2007;78(2):385-94. doi: 10.1093/Cvr/Cvm109.
33. Wagatsuma A, Tamaki H, Ogita F. Expression Of Vascular Endothelial Growth Factor And Its Receptors In Heart Tissue Following Short-Term Swimming Training. Int J Sport Health Sci. 2005;3:91-9. doi: 10.5432/Ijshs.3.91.
34. Soltani R, Gaeini A, Nuri R. The Effects Of 8 Weeks Aerobic Training On HIF-1α, Mir-21 And VEGF Gene Expression In Female Balb/C With Breast Cancer. Yafteh. 2019;21(1), [In Persian].
35. Shirali S, Barari A, Hosseini SA, Khodadi E. Effects Of Six Weeks Endurance Training And Aloe Vera Supplementation On COX-2 And VEGF Levels In Mice With Breast Cancer. Asian Pac J Cancer Prev: APJCP. 2017;18(1):31. doi: 10.22034/APJCP.2017.18.1.31.
36. Wang C-A, Harrell JC, Iwanaga R, Jedlicka P, Ford HL. Vascular Endothelial Growth Factor C Promotes Breast Cancer Progression Via A Novel Antioxidant Mechanism That Involves Regulation Of Superoxide Dismutase 3. Breast Cancer Res. 2014;16(5):462. doi: 10.1186/S13058-014-0462-2.
37. Mahrou M., Gaeini AA., Chobbineh S., Javidi M., (2000). Changes In Stimulating Factors Of Angiogenesis, Induced By Progressive Resistance Training In Diabetic Rats, Iran J Diabetes Lipid Disord. 2000;14(1):1-8 [In Persian].
38. Rios JL, Boldt KR, Mather JW, Seerattan RA, Hart DA, Herzog W. Quantifying The Effects Of Different Treadmill Training Speeds And Durations On The Health Of Rat Knee Joints. Sports Med Open. 2018;4(1):15. doi: 10.1186/S40798-018-0127-2.
39. Schulze‐Tanzil G, Al‐Sadi O, Wiegand E, Ertel W, Busch C, Kohl B, Et Al. The Role Of Pro‐Inflammatory And Immunoregulatory Cytokines In Tendon Healing And Rupture: New Insights. Scand J Med Sci Sports. 2011;21(3):337-51. doi: 10.1111/J.1600-0838.2010.01265.X.
40. Mirdar S, Hedayati M, Hajizade A. The Effect Of Endurance Swimming Exercise On HIF-1 Levels In Livers Of Pregnant Rats Exposed To Cadmium Toxicity. J Rafsanjan Univ Med Sci. 2014;12(11):919-28, [In Persian].
41. Songstad NT, Kaspersen K-HF, Hafstad AD, Basnet P, Ytrehus K, Acharya G. Effects Of High Intensity Interval Training On Pregnant Rats, And The Placenta, Heart And Liver Of Their Fetuses. PLoS One. 2015;10(11). doi:10.1371/Journal.Pone.0143095.
42. Abe T, Kitaoka Y, Kikuchi DM, Takeda K, Numata O, Takemasa T. High-Intensity Interval Training-Induced Metabolic Adaptation Coupled With An Increase In Hif-1α And Glycolytic Protein Expression. J Appl Physiol. 2015;119(11):1297-302. doi: 10.1152/Japplphysiol.00499.2015.
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Kheradmand S, Asad M R, Mir Javadi , R, Kheradmand N, Fashi M. Influence of Exercise Intensity on the Expression of Angiogenesis-Related Genes in the Hearts of Male Rats. Journal of Basic Research in Medical Sciences 2023; 10 (4) :43-53
URL: http://jbrms.medilam.ac.ir/article-1-495-en.html


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Volume 10, Issue 4 (12-2023) Back to browse issues page
مجله ی تحقیقات پایه در علوم پزشکی Journal of Basic Research in Medical Sciences
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