[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
About Journal::
Editorial Board::
Articles Archive::
Indexing Databases::
To Authors::
To Reviewers::
Submit Your Article::
Policies and Publication Ethics::
Archiving Policy::
Site Facilities::
Contact Us::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
Registered in



:: Volume 7, Issue 4 (9-2020) ::
2020, 7(4): 43-52 Back to browse issues page
Molecular identification of Dicrocoelium dendriticum using 28s rDNA genomic marker and its histopathologic features in domestic animals in western Iran
Mohammad Hossein Ebrahim Pour , Bahar Shemshadi , Alimohammad Bahrami , Salome Shirali
Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran , bshemshadi@yahoo.com
Abstract:   (1907 Views)
Introduction: Dicrocoeliasis is a common disease of bile ducts and gallbladder of domestic and wild ruminants. This disease is caused by different species of dicrocoelium including Dicrocoelium dendriticum. The aim of this study was to identify pathological damages and molecular features associated with this parasite in ruminants.
Materials and Methods: In this cross-sectional study, 180 fresh adult D. dendriticum worms were collected from 45 infected livers of slaughtered cattle, sheep, and goats in three western provinces of Iran. After histopathological examination, a 963 bp fragment (28S rRNA [MRT1] gene) was amplified using Polymerase Chain Reaction (PCR). The amplified fragment was digested by Trul1 restriction enzyme to evaluate through Restriction Fragment Length Polymorphism (RFLP) method.
 Results: Based on histological examination, hyperplasia of mucosal glands and swelling of bile ducts of infected livers were observed. In all studied samples, a 963-bp fragment was produced by PCR. Based on PCR-RFLP, the PCR products were digested by Trul1 restriction enzyme producing four fragments (116 bp, 145 bp, 293 bp, and 409 bp) all of which were related to D. dendriticum. Sequencing of the obtained 28S rDNA fragments showed that all of them were completely similar, and comparing them with the Gene Bank sequences showed 97% to 100% similarity between homogeneous fragments. The obtained sequences were registered in the Gene Bank with the accession numbers of MT539114, MT539115, and MT539116.
Conclusion: According to the present study, D. dendriticum is the predominant parasite infecting ruminants in west of Iran.

 [MRT1]DNA or RNA?
Keywords: Dicrocoelium dendriticum, Pathology, 28s rDNA, PCR-RLFP, Sequencing
Full-Text [PDF 407 kb]   (889 Downloads)    
Type of Study: Research | Subject: Parasitologiy
Received: 2020/04/2 | Accepted: 2020/08/27 | Published: 2020/09/19
Send email to the article author

Add your comments about this article
Your username or Email:


XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ebrahim Pour M H, Shemshadi B, Bahrami A, Shirali S. Molecular identification of Dicrocoelium dendriticum using 28s rDNA genomic marker and its histopathologic features in domestic animals in western Iran. Journal of Basic Research in Medical Sciences 2020; 7 (4) :43-52
URL: http://jbrms.medilam.ac.ir/article-1-535-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 7, Issue 4 (9-2020) Back to browse issues page
مجله ی تحقیقات پایه در علوم پزشکی Journal of Basic Research in Medical Sciences
Persian site map - English site map - Created in 0.16 seconds with 41 queries by YEKTAWEB 4654