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:: Volume 7, Issue 4 (9-2020) ::
JBRMS 2020, 7(4): 31-35 Back to browse issues page
Multilocus Sequence Typing (MLST) of Enterococcus faecalis clinical isolates in Ilam, Iran
Farzad Mohammadi , Jalil Vand Yousefi , Naser Harzandi , Sobhan Ghafourian
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran , sadeghgudarz@gmail.com
Abstract:   (1033 Views)
Introduction: The Enterococcus faecalis (E. faecalis) is the one of the pathogenic bacteria that become famous and considerable in the recent years. Here we tried to do typing the E. faecalis isolates to provide advantageous information that can help us to understand epidemiological communication between the E. faecalis isolates.
Materials and methods: One hundred  E. faecalis were isolated from urine samples of Imam Khomeini Hospital, Ilam, Iran. Afterwards, all isolates were confirmed by the phenotyping method and then for more certainty, every isolates were authenticated by PCR analysis of 16sRNA gene. Eventually, all isolates were considered as E. faecalis. For Multilocus Sequence Typing (MLST), 7 housekeeping genes were used to gain MLST scheme for epidemiological study. In addition, to determine various type of E. faecalis pubmlst database was selected and the MLST analysis was done based on recommended instruction by the pubMLST.org.
Results: The disk diffusion results demonstrated that fifty-four out of one hundred isolates were resistant, four isolates were semi sensitive and forty-two isolates were sensitive to vancomycin. So, 90 isolates were MLST. Using seven structural genes and using pubMLST.org database, different types of E. faecalis were determined. The MLST results demonstrated that 26 different group and Sequence Types (ST) obtained. Our findings demonstrated that the isolates were from different types.
Conclusion:  Accoeding to our results, we couldn't find any epidemic correlation between the isolates. Given that most of these isolates had resistance to vancomycin, they had low clonal correlation with each other and only had few similar STs pattern.
Keywords: E. faecalis, Multilocus Sequence Typing, Iran
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Type of Study: Research | Subject: Microbiology
Received: 2020/07/13 | Accepted: 2020/09/13 | Published: 2020/09/19
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Mohammadi F, Vand Yousefi J, Harzandi N, Ghafourian S. Multilocus Sequence Typing (MLST) of Enterococcus faecalis clinical isolates in Ilam, Iran. JBRMS 2020; 7 (4) :31-35
URL: http://jbrms.medilam.ac.ir/article-1-545-en.html

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Volume 7, Issue 4 (9-2020) Back to browse issues page
مجله ی تحقیقات پایه در علوم پزشکی Journal of Basic Research in Medical Sciences
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