Journal of Basic Research in Medical Sciences
Volume 10, Issue 1 (1-2023)
jbrms 2023, 10(1): 25-30 |
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Mohammadzadeh Zangalani H, Khalilzadeh Kaleybar S B. Influence of a single nucleotide polymorphism in miR-196a2 on idiopathic asthenozoospermia in Iranian Azeri males. jbrms 2023; 10 (1) :25-30
URL: http://jbrms.medilam.ac.ir/article-1-585-en.html
URL: http://jbrms.medilam.ac.ir/article-1-585-en.html
Department of Pathology, Tabriz Branch, Islamic Azad University, Tabriz, Iran , b_khalifehzadeh@yahoo.com
Abstract: (1316 Views)
Introduction: Asthenozoospermia (AZS), sperm immobility, is one of the major cause of men infertility. Evidence suggested that microRNAs (miRNAs) play a critical role in spermatogenesis process. However, the association of miRNAs polymorphisms with idiopathic male infertility remains unknown. Therefore, we investigated correlation between miR-196a2 rs11614913 polymorphism and idiopathic AZS among Iranian Azeri men.
Materials and Methods: In this study, 50 men with idiopathic AZS (case group) as well as 50 age and ethnically matched healthy men (control group) were enrolled from East Azerbaijan, Iran. The proteinase K method was used to extract the genomic DNA from sperm samples. Finally, genotyping was conducted using tetra-primer amplification refractory mutation system-polymerase chain reaction (Tetra-ARMS PCR) method.
Results: The frequency of TT, TC, and CC genotypes were 12%, 54%, and 34%, respectively, in patients with AZS; whereas the figures were 8%, 40%, and 52% in healthy controls, respectively. We found a significant difference between case and control groups in term of CC genotype frequency (P = 0.016).
Conclusion: We found a significant correlation between miR-196a2 rs11614913 polymorphism and AZS in Iranian Azeri men.
Materials and Methods: In this study, 50 men with idiopathic AZS (case group) as well as 50 age and ethnically matched healthy men (control group) were enrolled from East Azerbaijan, Iran. The proteinase K method was used to extract the genomic DNA from sperm samples. Finally, genotyping was conducted using tetra-primer amplification refractory mutation system-polymerase chain reaction (Tetra-ARMS PCR) method.
Results: The frequency of TT, TC, and CC genotypes were 12%, 54%, and 34%, respectively, in patients with AZS; whereas the figures were 8%, 40%, and 52% in healthy controls, respectively. We found a significant difference between case and control groups in term of CC genotype frequency (P = 0.016).
Conclusion: We found a significant correlation between miR-196a2 rs11614913 polymorphism and AZS in Iranian Azeri men.
Type of Study: Research |
Subject:
Medical genetics
Received: 2020/11/14 | Accepted: 2021/01/17 | Published: 2023/01/10
Received: 2020/11/14 | Accepted: 2021/01/17 | Published: 2023/01/10
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