[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
About Journal::
Editorial Board::
Articles Archive::
Indexing Databases::
To Authors::
To Reviewers::
Registration::
Submit Your Article::
Policies and Publication Ethics::
Archiving Policy::
Site Facilities::
Contact Us::
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations745605
h-index1110
i10-index1513
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE

..
:: Volume 10, Issue 3 (6-2023) ::
2023, 10(3): 51-61 Back to browse issues page
Effects of Aerobic Exercise Training, Pumpkin Seed Oil and Chickpea Supplementation on Gene Expression of Antioxidant Enzymes in the Liver and Muscle Tissues of Male Rats
Atiye Gholizadeh , Abbas Ghanbari-Niaki , Khadijeh Nasiri
Department of Exercise Physiology, Faculty of Sport Science, University of Mazandaran, Babolsar, Iran , abbas.ghanbari.umz@gmail.com
Abstract:   (243 Views)
Introduction: There is ample evidence regarding the protective effects of antioxidant vitamins and minerals against oxidative stress. The discovery of zinc's protective role in countering free-radical formation and oxidative stress has instigated extensive research into the antioxidant properties of zinc and its involvement in the antioxidant defense system. Furthermore, the utilization of plants with antioxidant properties has gained increasing attention among researchers. This study aims to investigate the impact of six weeks of aerobic exercise training, along with supplementation of pumpkin seed oil and chickpeas, on the gene expression of antioxidant enzymes in the liver and muscle tissues of male rats.
Material & Methods: To achieve this objective, 36 Wistar rats were divided into six groups: control (C), training (T), chickpea (Ch), chickpea + training (Ch+T), pumpkin seed oil (P), and pumpkin seed oil + training (P+T). At the conclusion of the training and supplementation period, the gene expression of the antioxidant enzymes glutathione peroxidase 1 (GPX1) and catalase (CAT) were assessed. Data were subjected to one-way analysis of variance at a significance level of P < 0.05.
Results: The results revealed that in all the groups involving pumpkin seed oil and chickpea supplementation, in both liver and muscle tissues, a non-significant decrease in the expression of the enzymes CAT and GPX1 was observed when compared to the control and exercise training groups.
Conclusion: To a certain extent, the use of pumpkin seed oil and chickpeas may enhance the expression of antioxidant enzymes.
Keywords: Zinc, Pumpkin seed oil, Chickpea, Catalase (CAT), Glutathione peroxidase (GPX)
Full-Text [PDF 3144 kb]   (69 Downloads)    
Type of Study: Research | Subject: Physiology
Received: 2022/03/2 | Accepted: 2022/03/15 | Published: 2023/06/25
References
1. Lee SR. Critical role of zinc as either an antioxidant or a prooxidant in cellular systems. Oxidative medicine and cellular longevity. 2018; Mar 20. doi: 10.1155/2018/9156285.
2. Chimienti F, Devergnas S, Pattou F, Schuit F, Garcia-Cuenca R, Vandewalle B, et al. In vivo expression and functional characterization of the zinc transporter ZnT8 in glucose-induced insulin secretion. Journal of cell science. 2006;119(20):4199-206. doi: 10.1242/jcs.03164.
3. Olgar Y, Ozdemir S, Turan BJM, Biochemistry C. Induction of endoplasmic reticulum stress and changes in expression levels of Zn2+-transporters in hypertrophic rat heart. Molecular and Cellular Biochemistry. 2018;440(1):209-19. doi: 10.1007/s11010-017-3168-9.
4. Prasad AS, Bao BJA. Molecular mechanisms of zinc as a pro-antioxidant mediator: clinical therapeutic implications. Antioxidants. 2019;8(6):164. doi: 10.3390/antiox8060164.
5. Brzóska MM, Kozłowska M, Rogalska J, Gałażyn-Sidorczuk M, Roszczenko A, Smereczański NMJN. Enhanced zinc intake protects against oxidative stress and its consequences in the brain: A study in an in vivo rat model of cadmium exposure. Nutrients. 2021;13(2):478. doi: 10.3390/nu13020478.
6. Madej D, Pietruszka B, Kaluza JJJoTEiM, Biology. The effect of iron and/or zinc diet supplementation and termination of this practice on the antioxidant status of the reproductive tissues and sperm viability in rats. Journal of Trace Elements in Medicine and Biology. 2021;64:126689. doi: 10.1016/j.jtemb.2020.126689.
7. Kara E, Gunay M, Cicioglu İ, Ozal M, Kilic M, Mogulkoc R, et al. Effect of zinc supplementation on antioxidant activity in young wrestlers. Biological trace element research. 2010;134(1):55-63. doi: 10.1007/s12011-009-8457-z.
8. Taherkhani S, Valaei K, Arazi H, Suzuki KJA. An overview of physical exercise and antioxidant supplementation influences on skeletal muscle oxidative stress. Antioxidants. 2021;10(10):1528. doi: 10.3390/antiox10101528.
9. Akomolafe SF. Effects of roasting on the phenolic phytochemicals and antioxidant activities of pumpkin seed. Vegetos. 2021;34(3):505-14. doi:10.1007/s42535-021-00226-w.
10. Bakeer MR, Saleh SY, Gazia N, Abdelrahman HA, Elolimy A, Abdelatty AMJIJoAS. Effect of dietary pumpkin (Cucurbita moschata) seed oil supplementation on reproductive performance and serum antioxidant capacity in male and nulliparous female V-Line rabbits. Italian Journal of Animal Science. 2021;20(1):419-25. doi: 10.1080/1828051X.2021.1889406.
11. Prommaban A, Kuanchoom R, Seepuan N, Chaiyana WJP. Evaluation of Fatty Acid Compositions, Antioxidant, and Pharmacological Activities of Pumpkin (Cucurbita moschata) Seed Oil from Aqueous Enzymatic Extraction. Plants. 2021;10(8):1582. doi: 10.3390/plants10081582.
12. Tüfekci B, Banjanin T, Lemiasheuski V, Özcan MM. A Review: Some composition properties of pumpkin seed and oil and its effect on human health. Journal of Agroalimentary Processes & Technologies. 2021 Jul 1;27(3).
13. Kaur R, Prasad K. Technological, processing and nutritional aspects of chickpea (Cicer arietinum)-A review. Trends in Food Science & Technology. 2021 Mar 1;109:448-63. doi: 10.1016/j.tifs.2021.01.044.
14. Wang J, Li Y, Li A, Liu RH, Gao X, Li D, Kou X, Xue Z. Nutritional constituent and health benefits of chickpea (Cicer arietinum L.): A review. Food Research International. 2021 Dec 1;150:110790. doi: 10.1016/j.foodres.2021.110790.
15. Faghfouri AH, Zarezadeh M, Aghapour B, Izadi A, Rostamkhani H, Majnouni A, Abu-Zaid A, Varkaneh HK, Ghoreishi Z, Ostadrahimi A. Clinical efficacy of zinc supplementation in improving antioxidant defense system: A comprehensive systematic review and time-response meta-analysis of controlled clinical trials. European Journal of Pharmacology. 2021 Sep 15;907:174243. doi: 10.1016/j.ejphar.2021.174243.
16. Howarth FC, Almugaddum FA, Qureshi MA, Ljubisavijevic M. Effects of varying intensity exercise on shortening and intracellular calcium in ventricular myocytes from streptozotocin (STZ)-induced diabetic rats. Molecular and cellular biochemistry. 2008 Oct;317:161-7. doi: 10.1007/s11010-008-9844-z.
17. Ghanbari Niaki A. Effect of an interval progressive (pyramidal) aerobic training on lipid and lipoprotein profiles in San Shou Athletes. The International Journal of Humanities. 2007 Jan 10;12(1):97-110.
18. Nobari H, Nejad HA, Kargarfard M, Mohseni S, Suzuki K, Carmelo Adsuar J, Pérez-Gómez J. The effect of acute intense exercise on activity of antioxidant enzymes in smokers and non-smokers. Biomolecules. 2021 Jan 27;11(2):171. doi: 10.3390/biom11020171.
19. Martínez-Noguera FJ, Alcaraz PE, Ortolano-Ríos R, Dufour SP, Marín-Pagán C. Differences between professional and amateur cyclists in endogenous antioxidant system profile. Antioxidants. 2021 Feb 12;10(2):282. doi: 10.3390/antiox10020282.
20. Thirupathi A, Wang M, Lin JK, Fekete G, István B, Baker JS, Gu Y. Effect of different exercise modalities on oxidative stress: a systematic review. BioMed Research International. 2021 Feb 11;2021:1-0. doi: 10.1155/2021/1947928.
21. Arazi H, Eghbali E, Suzuki K. Creatine supplementation, physical exercise and oxidative stress markers: A review of the mechanisms and effectiveness. Nutrients. 2021 Mar 6;13(3):869. doi: 10.3390/nu13030869.
22. Al-Ansari RF, Al-Gebori AM, Sulaiman GM. Serum levels of zinc, copper, selenium and glutathione peroxidase in the different groups of colorectal cancer patients. Caspian Journal of Internal Medicine. 2020;11(4):384. doi: 10.22088/cjim.11.4.384.
23. Peng M, Lu D, Liu J, Jiang B, Chen J. Effect of roasting on the antioxidant activity, phenolic composition, and nutritional quality of pumpkin (Cucurbita pepo L.) seeds. Frontiers in Nutrition. 2021 Mar 10;8:647354. doi: 10.3389/fnut.2021.647354.
24. Rabeh NM, Haggag MH, Mohammed AZ. Effect of corn silk, Pumpkin seeds, and creatine on oxidative stress of male exercise rats. doi: 10.21608/ejas.2020.145883.
25. Shokohi Rad N, Bagherpour T, Nemati N, Hojati V. The effect of pumpkin seed and endurance training on oxidative stress factors and DNA damage. Daneshvar Medicine. 2020 Aug 26;28(3):28-41.
26. Eraslan G, Kanbur M, Aslan Ö, Karabacak M. The antioxidant effects of pumpkin seed oil on subacute aflatoxin poisoning in mice. Environmental toxicology. 2013 Dec;28(12):681-8.. doi: 10.1002/tox.20763.
27. Mahbub R, Francis N, Blanchard C, Santhakumar A. The anti-inflammatory and antioxidant properties of chickpea hull phenolic extracts. Food Bioscience. 2021 Apr 1;40:100850. doi: 10.1016/j.fbio.2020.100850.
28. Aisa HA, Gao Y, Yili A, Ma Q, Cheng Z. Beneficial role of chickpea (Cicer arietinum L.) functional factors in the intervention of metabolic syndrome and diabetes mellitus. InBioactive food as dietary interventions for diabetes 2019 Jan 1 (pp. 615-627). Academic Press. doi: 10.1016/B978-0-12-813822-9.00039-4.
29. Soliman SS, Soliman AE. Ameliorative Effect of Ethanolic Extract from Cicer arietinum Seeds towards CCl4-Induced Liver Hepatotoxicity in Rats.
30. Poljsak B, Šuput D, Milisav I. Achieving the balance between ROS and antioxidants: when to use the synthetic antioxidants. Oxidative medicine and cellular longevity. 2013 Oct;2013. doi: 10.1155/2013/956792.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Gholizadeh A, Ghanbari-Niaki A, Nasiri K. Effects of Aerobic Exercise Training, Pumpkin Seed Oil and Chickpea Supplementation on Gene Expression of Antioxidant Enzymes in the Liver and Muscle Tissues of Male Rats. Journal of Basic Research in Medical Sciences 2023; 10 (3) :51-61
URL: http://jbrms.medilam.ac.ir/article-1-674-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 10, Issue 3 (6-2023) Back to browse issues page
مجله ی تحقیقات پایه در علوم پزشکی Journal of Basic Research in Medical Sciences
Persian site map - English site map - Created in 0.16 seconds with 41 queries by YEKTAWEB 4654