The Effect of Moderate Intensity Continuous Training (MICT) on TNF-α and Fetuin A in Type 2 Diabetic Wistar Rats

Vahdati , Tahereh; Soori , Rahman; Akbarnezhad , Ali; Pournemati , Parisa

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Volume 11, Issue 1 (1-2024)

2024, 11(1): 22-33

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The Effect of Moderate Intensity Continuous Training (MICT) on TNF-α and Fetuin A in Type 2 Diabetic Wistar Rats

Tahereh Vahdati

, Rahman Soori

, Ali Akbarnezhad

, Parisa Pournemati

Department of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran , Soori@ut.ac.ir

Abstract: (580 Views)

Introduction: Hepatokines secreted by the liver play a pivotal role in the pathogenesis of type 2 diabetes, directly influencing glucose and lipid metabolism. Exercise training is recognized as an effective treatment strategy for type 2 diabetes. This study aims to investigate the impact of moderate intensity continuous training (MICT) on the levels of Fetuin-A and tumor necrosis factor-alpha (TNF-α) in type 2 diabetic rats.
Material & Methods: Fifteen male Wistar rats aged between 8 and 10 weeks were randomly assigned to three groups (5 rats in each group): healthy control, diabetic, and training diabetic. Diabetes was induced through intraperitoneal injection of nicotinamide and streptozotocin (STZ). The MICT protocol involved eight weeks of continuous running, five days per week, at 55-60% of maximal oxygen consumption. Blood samples were collected 48 hours after the last training session, and serum levels of TNF-α and Fetuin-A were measured.
Results: The observed reductions in Fetuin-A, TNF-α, and glucose levels were not statistically significant in the trained group compared to the diabetic group. However, a significant decrease in insulin levels (p=0.002) and insulin resistance (p=0.01) was observed in the trained group compared to the diabetic group.
Conclusion: In conclusion, moderate intensity continuous training, as a non-pharmacological intervention, appears to play an effective role in the management of type 2 diabetes by improving insulin resistance and reducing insulin levels.

Keywords: Moderate Intensity Continuous Training (MICT), Fetuin-A, Tumor Necrosis Factor Alpha (TNF-α)

Full-Text [PDF 296 kb] (157 Downloads)

Type of Study: Research | Subject: Physiology
Received: 2023/02/8 | Accepted: 2023/02/13 | Published: 2024/01/20

References

1. Wang Y, Koh WP, Jensen MK, Yuan JM, Pan A. Plasma fetuin-A levels and risk of type 2 diabetes mellitus in a Chinese population: a nested case-control study. Diabetes Metab J. 2019; 43(4):474-86. doi: 10.4093/dmj.2018.0171.

2. Pal D, Dasgupta S, Kundu R, Maitra S, Das G, Mukhopadhyay S, Ray S, Majumdar SS, Bhattacharya S. Fetuin-A acts as an endogenous ligand of TLR4 to promote lipid-induced insulin resistance. Nat Med. 2012; 18(8):1279-85. doi: 10.1038/nm.2851.

3. Ix JH, Wassel CL, Kanaya AM, Vittinghoff E, Johnson KC, Koster A, et al. Fetuin-A and incident diabetes mellitus in older persons. JAMA. 2008; 300(2):182-8. doi: 10.1001/jama.300.2.182.

4. Jung TW, Youn BS, Choi HY, Lee SY, Hong HC, Yang SJ, et al. Salsalate and adiponectin ameliorate hepatic steatosis by inhibition of the hepatokine fetuin-A. Biochem Pharmacol. 2013; 86(7):960-9. doi: 10.1016/j.bcp.2013.07.034.

5. Sindhu S, Akhter N, Shenouda S, Wilson A, Ahmad R. Plasma fetuin-A/α2-HS-glycoprotein correlates negatively with inflammatory cytokines, chemokines and activation biomarkers in individuals with type-2 diabetes. BMC Immunol. 2016; 17(1):33. doi: 10.1186/s12865-016-0171-y.

6. Jung CH, Kim BY, Kim CH, Kang SK, Jung SH, Mok JO. Associations of serum fetuin-A levels with insulin resistance and vascular complications in patients with type 2 diabetes. Diab Vasc Dis Res. 2013; 10(5):459-67. doi: 10.1177/1479164113490766.

7. Skrypnik D, Ratajczak M, Karolkiewicz J, Mądry E, Pupek-Musialik D, Hansdorfer-Korzon R, et al. Effects of endurance and endurance–strength exercise on biochemical parameters of liver function in women with abdominal obesity. Biomed Pharmacother. 2016; 80:1-7. doi: 10.1016/j.biopha.2016.02.017.

8. Kirwan JP, Sacks J, Nieuwoudt S. The essential role of exercise in the management of type 2 diabetes. Cleve Clin J Med. 2017; 84(7 1): 15-21. doi: 10.3949/ccjm.84. s1.03.

9. Gibala MJ, Little JP, MacDonald MJ, Hawley JA. Physiological adaptations to low‐volume, high‐intensity interval training in health and disease. J Physiol. 2012; 590(5):1077-84. doi: 10.1113/jphysiol.2011.224725.

10. Malin SK, Mulya A, Fealy CE, Haus JM, Pagadala MR, Scelsi AR, et al. Fetuin-A is linked to improved glucose tolerance after short-term exercise training in nonalcoholic fatty liver disease. J Appl Physiol. 2013; 115(7):988-94. doi: 10.1152/japplphysiol.00237.2013.

11. Zhang LY, Liu T, Teng YQ, Yao XY, Zhao TT, Lin LY, et al. Effect of a 12-week aerobic exercise training on serum fetuin-A and adipocytokine levels in type 2 diabetes. Exp Clin Endocrinol Diabetes. 2018; 126(08):487-92. doi: 10.1055/s-0043-115904.

12. Misu H, Takamura T, Takayama H, Hayashi H, Matsuzawa-Nagata N, Kurita S, et al. A liver-derived secretory protein, selenoprotein P, causes insulin resistance. Cell Metab. 2010; 12(5):483-95. doi: 10.1016/j.cmet.2010.09.015.

13. Pietiläinen KH, Kaprio J, Borg P, Plasqui G, Yki‐Järvinen H, Kujala UM, et al. Physical inactivity and obesity: a vicious circle. Obesity. 2008; 16(2):409-14. doi: 10.1038/oby.2007.72.

14. El-shaer RA, Abd-Ellatif RN, El-Tabaa EF, Awad MM. Fetuin A Levels Among Different Grades Of Obesity With Its Potential Link To Its Complication With Elaboration Of Physical Training Effects In Rats. BESPS. 2022; 42(3):231-45. doi: 10.21608/BESPS.2022.111806.1115.

15. Swaroop JJ, Rajarajeswari D, Naidu JN. Association of TNF-α with insulin resistance in type 2 diabetes mellitus. Indian J Med Res. 2012; 135(1):127-30. doi: 10.4103/0971-5916.93435.

16. Pedersen BK, Toft AD. Effects of exercise on lymphocytes and cytokines. Br J Sports Med. 2000; 34(4):246-51. doi: 10.1136/bjsm.34.4.246.

17. Jorge ML, de Oliveira VN, Resende NM, Paraiso LF, Calixto A, Diniz AL, et al. The effects of aerobic, resistance, and combined exercise on metabolic control, inflammatory markers, adipocytokines, and muscle insulin signaling in patients with type 2 diabetes mellitus. Metabolism. 2011; 60(9):1244-52. doi: 10.1016/j.metabol.2011.01.006.

18. Powers SK, Criswell D, Lawler JO, Martin D, Lieu FK, Ji LL, Herb RA. Rigorous exercise training increases superoxide dismutase activity in ventricular myocardium. Am J Physiol. 1993; 265(6): 2094-8. doi: 10.1152/ajpheart.1993.265.6.H2094.

19. Mori K, Emoto M, Araki T, Yokoyama H, Lee E, Teramura M, et al. Effects of pioglitazone on serum fetuin-A levels in patients with type 2 diabetes mellitus. Metabolism. 2008; 57(9):1248-52. doi: 10.1016/j.metabol.2008.04.019.

20. Sakr HF, Al-Hashem FH, El-Naby WM, Alkhateeb MA, Zaki MS, Refaey HM, et al. Preventive roles of swimming exercise and pioglitazone treatment on hepatic dysfunction in a rat model of metabolic syndrome. Can J Physiol Pharmacol. 2014;92(2):162-70. doi: 10.1139/cjpp-2013-0043.

21. Keihanian A, Arazi H, Kargarfard M. Effects of aerobic versus resistance training on serum fetuin-A, fetuin-B, and fibroblast growth factor-21 levels in male diabetic patients. Physiol Int. 2019; 106(1):70-80. doi: 10.1556/2060.106.2019.01.

22. Schultes B, Frick J, Ernst B, Stefan N, Fritsche A. The effect of 6-weeks of aerobic exercise training on serum fetuin-A levels in non-diabetic obese women. Exp Clin Endocrinol Diabetes. 2010; 118(10):754-6. doi: 10.1055/s-0030-1253418.

23. Tönjes A, Fasshauer M, Kratzsch J, Stumvoll M, Blüher M. Adipokine pattern in subjects with impaired fasting glucose and impaired glucose tolerance in comparison to normal glucose tolerance and diabetes. PloS one. 2010; 5(11): e13911. doi: 10.1371/journal.pone.0013911.

24. Malin SK, Haus JM, Solomon TP, Blaszczak A, Kashyap SR, Kirwan JP. Insulin sensitivity and metabolic flexibility following exercise training among different obese insulin-resistant phenotypes. Am J Physiol Endocrinol Metab. 2013; 305(10): 1292-8. doi: 10.1152/ajpendo.00441.2013.

25. Diack SL. The determination of the surface area of the white rat. J Nutr. 1930; 3(3):289-96. doi: 10.1152/ajplegacy.1929.89.1.24.

26. Bradley RL, Jeon JY, Liu FF, Maratos-Flier E. Voluntary exercise improves insulin sensitivity and adipose tissue inflammation in diet-induced obese mice. Am J Physiol Endocrinol Metab. 2008; 295(3): 586-94. doi: 10.1152/ajpendo.00309.2007.

27. Malin SK, Del Rincon JP, Huang H, Kirwan JP. Exercise-induced lowering of fetuin-A may increase hepatic insulin sensitivity. Med Sci Sports Exerc. 2014; 46(11):2085-90. doi: 10.1249/MSS.0000000000000338.

28. Qiu J, Yuan H, Chen S, Zhou Y, Song D, Chen R. TNF α up-regulates COX-2 in chronic progressive nephropathy through nuclear accumulation of RelB and NF-κ B2. Arch Physiol Biochem. 2016; 122(2):88-93. doi: 10.3109/13813455.2016.1141961.

29. Mathews ST, Rakhade S, Zhou X, Parker GC, Coscina DV, Grunberger G. Fetuin-null mice are protected against obesity and insulin resistance associated with aging. Biochem Biophys Res Commun. 2006; 350(2):437-43. doi: 10.1016/j.bbrc.2006.09.071.

30. Dasgupta S, Bhattacharya S, Biswas A, Majumdar SS, Mukhopadhyay S, Ray S, et al. NF-κB mediates lipid-induced fetuin-A expression in hepatocytes that impairs adipocyte function effecting insulin resistance. Biochem J. 2010; 429(3):451-62. doi: 10.1042/BJ20100330.

31. Cartee GD, Funai K. Exercise and insulin: Convergence or divergence at AS160 and TBC1D1? Exerc Sport Sci Rev. 2009; 37(4):188-95. doi: 10.1097/JES.0b013e3181b7b7c5.

32. Kirwan JP, Del Aguila LF, Hernandez JM, Williamson DL, O'Gorman DJ, et al. Regular exercise enhances insulin activation of IRS-1-associated PI3-kinase in human skeletal muscle. J Appl Physiol. 2000; 88(2):797-803. doi: 10.1152/jappl.2000.88.2.797.

33. Mukhopadhyay S, Mondal SA, Kumar M, Dutta D. Proinflammatory and antiinflammatory attributes of fetu Iν-A: a novel hepatokine modulating cardiovascular and glycemic outcomes in metabolic syndrome. Endocr Pract. 2014; 20(12):1345-51. doi: 10.4158/EP14421.RA.

34. Azarbayjani MA, Banaeifar A, Arshadi S. The Effect of Aerobic Training and Adenosine on the Expression of SREBP-1C and A1 Receptor in Hepatic Fat-fed Rats. Iran J Nutr Sci. Food Technol. 2019; 14(1):1-9.

35. Oh KJ, Lee DS, Kim WK, Han BS, Lee SC, Bae KH. Metabolic adaptation in obesity and type II diabetes: myokines, adipokines and hepatokines. Int J Mol Sci. 2016; 18(1):8. doi: 10.3390/ijms18010008.

36. Goldberg RB. Cytokine and cytokine-like inflammation markers, endothelial dysfunction, and imbalanced coagulation in development of diabetes and its complications. J Clin Endocrinol Metab. 2009; 94(9):3171-82. doi: 10.1210/jc.2008-2534.

37. Karstoft K, Pedersen BK. Exercise and type 2 diabetes: focus on metabolism and inflammation. Immunol Cell Biol. 2016; 94(2):146-50. doi: 10.1038/icb.2015.101.

38. Plomgaard P, Bouzakri K, Krogh-Madsen R, Mittendorfer B, Zierath JR, Pedersen BK. Tumor necrosis factor-α induces skeletal muscle insulin resistance in healthy human subjects via inhibition of Akt substrate 160 phosphorylation. Diabetes. 2005; 54(10):2939-45. doi: 10.2337/diabetes.54.10.2939.

39. Rajkovic N, Zamaklar M, Lalic K, Jotic A, Lukic L, Milicic T, et al. Relationship between obesity, adipocytokines and inflammatory markers in type 2 diabetes: relevance for cardiovascular risk prevention. Int J Environ Res Public Health. 2014; 11(4):4049-65. doi: 10.3390/ijerph110404049.

40. Chen X, Sun X, Wang C, He H. Effects of exercise on inflammatory cytokines in patients with type 2 diabetes: a meta-analysis of randomized controlled trials. Oxid Med Cell Longev. 2020. doi: 10.1155/2020/6660557.

41. Martin-Cordero L, García JJ, Hinchado MD, Bote E, Manso R, Ortega E. Habitual physical exercise improves macrophage IL-6 and TNF-α deregulated release in the obese Zucker rat model of the metabolic syndrome. Neuroimmunomodulation. 2011;18(2):123-30. doi: 10.1159/000322053.

42. Kadoglou NP, Iliadis F, Angelopoulou N, Perrea D, Ampatzidis G, Liapis CD, Alevizos M. The anti-inflammatory effects of exercise training in patients with type 2 diabetes mellitus. Eur J Cardiovasc Prev Rehabil. 2007; 14(6):837-43. doi: 10.1097/HJR.0b013e3282efaf50.

43. Isanejad A, HasanSarraf Z, Mahdavi M, Gharakhanlou R. The effect of aerobic exercise training on serum levels of TNF-α, IL-1β, IL-6 and Hsp70 in rats. J Sport Biosci. 2013; 4(15):91-106. doi: 10.22059/jsb.2013.29780.

44. Hennige AM, Staiger H, Wicke C, Machicao F, Fritsche A, Häring HU, et al. Fetuin-A induces cytokine expression and suppresses adiponectin production. PloS one. 2008; 3(3): e1765. doi: 10.1371/journal.pone.0001765.

45. Jung CH, Kim BY, Kim CH, Kang SK, Jung SH, Mok JO. Associations of serum fetuin-A levels with insulin resistance and vascular complications in patients with type 2 diabetes. Diab Vasc Dis Res. 2013; 10(5):459-67. doi: 10.1177/1479164113490766.

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Vahdati T, Soori R, Akbarnezhad A, Pournemati P. The Effect of Moderate Intensity Continuous Training (MICT) on TNF-α and Fetuin A in Type 2 Diabetic Wistar Rats. Journal of Basic Research in Medical Sciences 2024; 11 (1) :22-33
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