Comparison of Cluster Differentiation 44 expression in Oral Lichen Planus, Oral Leukoplakia and Oral Squamous Cell Carcinoma

Mostafazadeh, Samira; Tayefeh Maleki, Ata; Roozegar, Mohamad Ali; Abdal, Fariba

[Home ] [Archive]

Journal of Basic Research in Medical Sciences

Main Menu

Home

About Journal

Editorial Board

Articles Archive

Indexing Databases

To Authors

To Reviewers

Registration

Submit Your Article

Policies and Publication Ethics

Archiving Policy

Site Facilities

Contact Us

Google Scholar Metrics

	All	Since 2019
Citations	804	666
h-index	12	11
i10-index	17	14

Search in website

Receive site information

Registered in

Volume 12, Issue 1 (1-2025)

2025, 12(1): 17-25

Back to browse issues page

Comparison of Cluster Differentiation 44 expression in Oral Lichen Planus, Oral Leukoplakia and Oral Squamous Cell Carcinoma

Samira Mostafazadeh

, Ata Tayefeh Maleki

, Mohamad Ali Roozegar

, Fariba Abdal

Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Ilam University of Medical Sciences, Ilam, Iran.

Abstract: (88 Views)

Objectives: The aim of the study is to compare the expression of CD44 in OLP, OL and OSCC. Methods and Materials: This laboratory study is performed on paraffin blocks from tissue biopsies of patients with OLP, OL and OSCC available in the maxillofacial archive of pathology department of Imam Khomeini hospital, Urmia; 12 blocks of each lesion and 10 normal mucosal tissue blocks as a control group. The smears were stained by H&E and then stained by CD44 antibody kit and cut into 5-micron dimensions. 4 codes were used to evaluate the intensity of staining based on the average percentage of stained cells to total cells: staining less than five percent with a negative code, staining five to 25% code +1, staining 25% to 50 % code +2, staining 50% to 75% code +3 and for staining 75% and above code +4. Then the data was analyzed. Results: There is a significant difference between the three lesions of OLP, OL, and OSCC in the expression level of CD44 (p<0.001) and expression of this molecule in the mucosa of OSCC was higher compared to the OLP and OL. Also, the expression of CD44 in three lesions of OLP, OL, and OSCC had a statistically significant difference with oral normal mucosa (p<0.001). Conclusion: Based on the findings, this protein can probably be a suitable marker to confirm the prediction of dysplasia in oral premalignant lesions such as OLP and OL, and invasion, metastasis, prognosis and recurrence in OSCC.

Keywords: Oral Squamous Cell Carcinoma, Oral Lichen Planus, Oral leukoplakia, cd44

Full-Text [PDF 1821 kb] (50 Downloads)

Type of Study: Research | Subject: Medical Pathology
Received: 2024/03/5 | Accepted: 2024/10/21 | Published: 2025/01/12

References

1. Venkat Naga SKS, Shekar PC, Kattappagari KK, Prakash Chandra KL, Reddy GS, Ramana Reddy BV. Expression of cluster differentiation-44 stem cell marker in grades of oral epithelial dysplasia: A preliminary study. J Oral Maxillofac Pathol. 2019;23(2):203-7. doi: 10.4103/jomfp.JOMFP_308_18

2. Kaza S, Kantheti LP, Poosarla C, Gontu SR, Kattappagari KK, Baddam VR. A study on the expression of CD44 adhesion molecule in oral squamous cell carcinoma and its correlation with tumor histological grading. J Orofac Sci. 2018;10(1):42-9. doi: 10.4103/0973-029X.140722

3. Ghazi N, Saghravanian N, Taghi Shakeri M, Jamali M. Evaluation of CD44 and TGF-B Expression in Oral Carcinogenesis. J Dent (Shiraz). 2021;22(1):33-40. doi.org/10.30476/dentjods.2020.84393.1079

4. • 4- Saghravanian N, Anvari K, Ghazi N, Memar B, Shahsavari M, Aghaee MA. Expression of p63 and CD44 in oral squamous cell carcinoma and correlation with clinicopathological parameters. Arch Oral Biol. 2017;82:160-5. DOI: 10.1016/j.archoralbio.2017.06.011

5. Zargaran M, Baghaei F, Moghimbeigi A. Comparative study of β-catenin and CD44 immunoexpression in oral lichen planus and squamous cell carcinoma. Int J Dermatol. 2018;57(7):794-8. DOI: 10.1111/ijd.14007

6. González-Moles MÁ, Warnakulasuriya S, González-Ruiz I, González-Ruiz L, Ayén Á, Lenouvel D, Ruiz-Ávila I, Ramos-García P. Worldwide prevalence of oral lichen planus: A systematic review and meta-analysis. Oral Dis. 2021;27(4):813-828. doi.org/10.1111/odi.13323

7. Thongprasom K. Oral lichen planus: Challenge and management. Oral Dis. 2018;24(1-2):172-173. DOI: 10.1111/odi.12712

8. Glick M. Burket's oral medicine. PMPH USA. 2015.

9. Bánóczy J. Oral leukoplakia (Vol. 8). Springer Science & Business Media. 2012.

10. Kumar S1, Muniyandi M2. Tobacco use and oral leukoplakia: cross-sectional study among the Gond tribe in Madhya Prades . Asian Pac J Cancer Prev . 2015;16(4):1515-8. doi: 10.7314/apjcp.2015.16.4.1515.

11. Mustafa MB, Hassan MO, Alhussein A, Mamoun E, El Sheikh M, Suleiman AM. Oral leukoplakia in the Sudan: clinicopathological features and risk factors. Int Dent J. 2019;69(6):428-435. doi: 10.1111/idj.12509

12. • 12- Čēma I, Dzudzilo M, Kleina R, Franckevica I, Svirskis Š. Correlation of Soluble CD44 Expression in Saliva and CD44 Protein in Oral Leukoplakia Tissues. Cancers (Basel). 2021;13(22):5739. DOI: 10.3390/cancers13225739

13. Carrard VC, van der Waal I. A clinical diagnosis of oral leukoplakia; A guide for dentists. Med Oral Patol Oral Cir Bucal. 2018;23(1):e59-e64. doi: 10.4317/medoral.22292.

14. Boxberg M, Götz C, Haidari S, Dorfner C, Jesinghaus M, Drecoll E, Boskov M, Wolff KD, Weichert W, Haller B, Kolk A. Immunohistochemical expression of CD44 in oral squamous cell carcinoma in relation to histomorphological parameters and clinicopathological factors. Histopathology. 2018;73(4):559-72. doi.org/10.1111/his.13496

15. Yamada S, Itai S, Nakamura T, Yanaka M, Kaneko MK, Kato Y. Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5. Biochem Biophys Rep. 2018;14:64-8. DOI: 10.1016/j.bbrep.2018.03.007

16. Chen W, Zhou Y, Ma Z, Xie Y. Expressions of miR-590 in Oral Lichen Planus and Oral Squamous Cell Carcinoma Tissues and Clinical Values. J Hard Tissue Biol. 2021;30(4):363-70. doi.org/10.2485/jhtb.30.363

17. Rautava J, Soukka T, Inki P, Leimola-Virtanen R, Saloniemi I, Happonen RP, Heikinheimo K. CD44v6 in developing, dysplastic and malignant oral epithelia. Oral Oncol. 2003;39(4):373-9. doi: 10.1016/s1368-8375(02)00140-9.

18. Sawant S, Gokulan R, Dongre H, Vaidya M, Chaukar D, Prabhash K, Ingle A, Joshi S, Dange P, Joshi S, Singh AK, Makani V, Sharma S, Jeyaram A, Kane S, D'Cruz A. Prognostic role of Oct4, CD44 and c-Myc in radio-chemo-resistant oral cancer patients and their tumourigenic potential in immunodeficient mice. Clin Oral Investig. 2016;20(1):43-56. doi: 10.1007/s00784-015-1476-6.

19. Abdulmajeed AA, Dalley AJ, Farah CS. Putative cancer stem cell marker expression in oral epithelial dysplasia and squamous cell carcinoma. J Oral Pathol Med. 2013;42(10):755-60. doi: 10.1111/jop.12073

20. Mannelli G, Magnelli L, Deganello A, Busoni M, Meccariello G, Parrinello G, Gallo O. Detection of putative stem cell markers, CD44/CD133, in primary and lymph node metastases in head and neck squamous cell carcinomas. A preliminary immunohistochemical and in vitro study. Clin Otolaryngol. 2015;40(4):312-20. doi: 10.1111/coa.12368.

21. • 21- Simionescu C, Mărgăritescu C, Surpăţeanu M, Mogoantă L, Zăvoi R, Ciurea R, Surlin P, Stepan A. The study of E-cadherine and CD44 immunoexpression in oral squamous cell carcinoma. Rom J Morphol Embryol. 2008;49(2):189-93. PMID: 18516325

22. Misra S, Hascall VC, Markwald RR, Ghatak S. Interactions between hyaluronan and its receptors (CD44, RHAMM) regulate the activities of inflammation and cancer. Frontiers in immunology. 2015;6:201. doi.org/10.3389/fimmu.2015.00201

Add your comments about this article

Mendeley

Zotero

RefWorks

Mostafazadeh S, Tayefeh Maleki A, Roozegar M A, Abdal F. Comparison of Cluster Differentiation 44 expression in Oral Lichen Planus, Oral Leukoplakia and Oral Squamous Cell Carcinoma. Journal of Basic Research in Medical Sciences 2025; 12 (1) :17-25
URL: http://jbrms.medilam.ac.ir/article-1-836-en.html

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Volume 12, Issue 1 (1-2025)

Back to browse issues page

Persian site map - English site map - Created in 0.15 seconds with 41 queries by YEKTAWEB 4670