Introduction: Escherichia coli (E.coli) as a main cause of both nosocomial and community-acquired infections in humans have a relative potential to develop resistance. Nowadays, most infections caused by ESBL-producing E.coli (ESBLEC) had mostly been described as nosocomial acquired or nursing home related. In this study, we employed E-test assay to detect antibiotic resistance of E.coli strains and determine MIC of antibiotics.

Materials and methods: Thirty E.coli strains gathered from Imam Khomeini hospital of Ilam, and cultured on TSB and bacterial suspension prepared by 0.5 µF concentration for E-test. Mueller Hinton agar and E-test strips of Amikacin, Cefepime, Ceftazidime, Ceftriaxone, Gentamicin, Meropenem, Nitrofurantoin, Piperacillin/Tazobactam, Tetracycline, Ticarcillin/ Clavulanic acid, Tobramycin, Trimethoprim were used

Results: Resistance to Ceftriaxone, Tobramycin, Gentamicin, Ticarcillin/ Clavulanic, Amikacin were 19.8%, 26.4%, 23.3%, 62.7%, 70.3%, respectively.