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JBRMS 2017, 4(1): 30-36 Back to browse issues page
Effect of olfactory ensheathing cells (OECs) transplantation on functional recovery in acute phase of spinal contused rats
Somayeh Heidarizadi, Naser Abbasi, Khairollah Asadollahi, Sara Rezaei, Ardeshir Moayeri, Monireh Azizi
Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran , azizi.moaz@gmail.com
Abstract:   (3468 Views)

Introduction: Spinal cord injuries (SCI) lead to permanent irreversible functional deficits.  Poor prognosis of patients is the motivation of searching a treatment for the chronic injury.  Planting stem cells provides us with a promising strategy. In the meanwhile, the use of olfactory ensheathing cells (OECs) has shown very good results.  This study aims at evaluating the effect of transplanted OECs on functional recovery of acute SCI in rats.

Materials and methods: In this study, eighteen adult male Wistar rats weighting approximately 210 ± 10 gr were used to study spinal cord injury. They were randomly divided into four groups: Sham (n = 3), Control (n = 6), Vehicle (n=3) and Treatment (n=6). In the Sham Group, only laminectomy was performed in the area of T9 spinal cord. In three groups of Control, vehicle and Treatment, after laminectomy, spinal cord contusion model was performed using the Weight drop technique. Immediately after the injury, 10μl Dulbecco's Modified Eagle Medium (DMEM) alone or with 106 cells were injected to vehicle and Treatment Group animals. For culturing cells, the olfactory mucosa of 7-day-old male Wistar rats was used. Motor function of animals in all groups, was evaluated in the first 48 hours daily and then weekly for eight weeks.

Results: Comparing the results of the second to eighth week of the study showed significant differences in the group receiving the OECs with the control group (P<0.05).

Conclusion: The results indicate a positive influence of the olfactory ensheathing cells in functional improvement of spinal cord injury in the acute phase of injury.

Keywords: Spinal cord injury, Olfactory ensheathing cells
Full-Text [PDF 591 kb]   (1040 Downloads)    
Type of Study: Research | Subject: Medical Anatomy
Received: 2015/09/28 | Accepted: 2016/06/16 | Published: 2016/07/21
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Heidarizadi S, Abbasi N, Asadollahi K, Rezaei S, Moayeri A, Azizi M. Effect of olfactory ensheathing cells (OECs) transplantation on functional recovery in acute phase of spinal contused rats. JBRMS 2017; 4 (1) :30-36
URL: http://jbrms.medilam.ac.ir/article-1-192-en.html

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Volume 4, Issue 1 (1-2017) Back to browse issues page
مجله ی تحقیقات پایه در علوم پزشکی Journal of Basic Research in Medical Sciences
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