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:: Volume 11, Issue 1 (1-2024) ::
2024, 11(1): 45-57 Back to browse issues page
T helper1/T helper2 and Interferon-γ/Interleukin-10 Ratios in Liver Transplant Rejection
Sara Assadiasl , Mohsen Nassiri Toosi , Narjes Soleimanifar , Bahareh Mohebbi , Bita Ansaripour , Maryam Sadr , Hanieh Mojtahedi , Banafsheh Mosharmovahed , Fatemeh Fazeli , Mohammad Hossein Nicknam
Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran, Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran , mhnicknam@sina.tums.ac.ir
Abstract:   (661 Views)
Introduction: Immune monitoring in transplant recipients, examining lymphocyte subsets and cytokine levels, is pivotal for advancing individualized medicine in transplantation. This study aims to assess T helper 1 and 2 cells in acute liver transplant rejection.
Material & Methods: Thirty liver transplantation candidates were enrolled pre- and six months post-transplantation under stable condition. Additionally, fifteen recipients with acute rejection, matched for age and transplantation duration, were included. Flow cytometry and ELISA assessed TCD4+CXCR3+IFN-γ (T helper 1) and TCD4+CCR4+IL-4+ (Th2) cell frequencies, as well as serum IFN-γ and IL-10 levels.
Results: Stable recipients showed significant decreases in Th1 and Th2 cell percentages six months post-transplant (both p < 0.0001), maintaining a comparable Th1/Th2 ratio. Serum IFN-γ levels also decreased. Conversely, the rejection group exhibited higher Th1 cell proportions and increased IFN-γ concentration compared to stable recipients (P = 0.03 and 0.001, respectively). IL-10 levels slightly decreased in both groups. Consequently, the IFN-γ/IL-10 ratio significantly increased during acute rejection (p < 0.0001). Th1 cell frequency and IFN-γ levels negatively correlated with allograft function.
Conclusion: Six months post-transplant, stable recipients experienced decreased Th1 and Th2 cell percentages, maintaining Th1/Th2 balance. The IFN-γ/IL-10 ratio significantly increased during acute rejection, indicating an immunological imbalance. Elevated Th1 frequency and IFN-γ levels were negatively correlated with allograft function, emphasizing their potential role in rejection dynamics.
Keywords: Liver Transplantation, Graft Rejection, T-Lymphocytes, Interleukin-10, Interferon-gamma
Full-Text [PDF 1062 kb]   (127 Downloads)    
Type of Study: Research | Subject: Medical immunology
Received: 2023/10/23 | Accepted: 2024/02/15 | Published: 2024/03/20
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Assadiasl S, Nassiri Toosi M, Soleimanifar N, Mohebbi B, Ansaripour B, Sadr M, et al . T helper1/T helper2 and Interferon-γ/Interleukin-10 Ratios in Liver Transplant Rejection. Journal of Basic Research in Medical Sciences 2024; 11 (1) :45-57
URL: http://jbrms.medilam.ac.ir/article-1-782-en.html
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Volume 11, Issue 1 (1-2024) Back to browse issues page
مجله ی تحقیقات پایه در علوم پزشکی Journal of Basic Research in Medical Sciences
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