[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
About Journal::
Editorial Board::
Articles Archive::
Indexing Databases::
To Authors::
To Reviewers::
Registration::
Submit Your Article::
Policies and Publication Ethics::
Archiving Policy::
Site Facilities::
Contact Us::
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations745605
h-index1110
i10-index1513
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE

..
:: Volume 11, Issue 1 (1-2024) ::
2024, 11(1): 45-57 Back to browse issues page
T helper1/T helper2 and Interferon-γ/Interleukin-10 Ratios in Liver Transplant Rejection
Sara Assadiasl , Mohsen Nassiri Toosi , Narjes Soleimanifar , Bahareh Mohebbi , Bita Ansaripour , Maryam Sadr , Hanieh Mojtahedi , Banafsheh Mosharmovahed , Fatemeh Fazeli , Mohammad Hossein Nicknam
Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran, Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran , mhnicknam@sina.tums.ac.ir
Abstract:   (342 Views)
Introduction: Immune monitoring in transplant recipients, examining lymphocyte subsets and cytokine levels, is pivotal for advancing individualized medicine in transplantation. This study aims to assess T helper 1 and 2 cells in acute liver transplant rejection.
Material & Methods: Thirty liver transplantation candidates were enrolled pre- and six months post-transplantation under stable condition. Additionally, fifteen recipients with acute rejection, matched for age and transplantation duration, were included. Flow cytometry and ELISA assessed TCD4+CXCR3+IFN-γ (T helper 1) and TCD4+CCR4+IL-4+ (Th2) cell frequencies, as well as serum IFN-γ and IL-10 levels.
Results: Stable recipients showed significant decreases in Th1 and Th2 cell percentages six months post-transplant (both p < 0.0001), maintaining a comparable Th1/Th2 ratio. Serum IFN-γ levels also decreased. Conversely, the rejection group exhibited higher Th1 cell proportions and increased IFN-γ concentration compared to stable recipients (P = 0.03 and 0.001, respectively). IL-10 levels slightly decreased in both groups. Consequently, the IFN-γ/IL-10 ratio significantly increased during acute rejection (p < 0.0001). Th1 cell frequency and IFN-γ levels negatively correlated with allograft function.
Conclusion: Six months post-transplant, stable recipients experienced decreased Th1 and Th2 cell percentages, maintaining Th1/Th2 balance. The IFN-γ/IL-10 ratio significantly increased during acute rejection, indicating an immunological imbalance. Elevated Th1 frequency and IFN-γ levels were negatively correlated with allograft function, emphasizing their potential role in rejection dynamics.
Keywords: Liver Transplantation, Graft Rejection, T-Lymphocytes, Interleukin-10, Interferon-gamma
Full-Text [PDF 2191 kb]   (74 Downloads)    
Type of Study: Research | Subject: Medical immunology
Received: 2023/10/23 | Accepted: 2024/01/15 | Published: 2024/01/20
References
1. Zarrinpar A, Busuttil RW. Liver transplantation: past, present and future. Nat Rev Gastroenterol Hepatol. 2013;10(7):434-40. doi:10.1038/nrgastro.2013.88
2. Moini M, Schilsky ML, Tichy EM. Review on immunosuppression in liver transplantation. World J Hepatol. 2015;7(10):1355. doi: 10.4254/wjh.v7.i10.1355
3. Baumann AK, Schlue J, Noyan F, Hardtke‐Wolenski M, Lehner F, Barg‐Hock H, et al. Preferential accumulation of T helper cells but not cytotoxic T cells characterizes benign subclinical rejection of human liver allografts. Liver Transpl. 2016;22(7):943-55. [DOI:10.1002/lt.24427]
4. Sood S, Testro AG. Immune monitoring post liver transplant. World J Transplant. 2014;4(1):30. doi: 10.5500/wjt.v4.i1.30
5. Wang Y, Tang Z, Gao W, Jiang Y, Peng L. Influence of Th1, Th2, and Th3 cytokines during the early phase after liver transplantation. Transplant Proc. 2003. Dec;35(8):3024-5. doi: 10.1016/j.transproceed. 2003.10.007.
6. Ganschow R, Broering D, Nolkemper D, Albani J, Kemper M, Rogiers X, et al. Th2 cytokine profile in infants predisposes to improved graft acceptance after liver transplantation. Transplantation. 2001;72(5):929-34. doi: 10.1097/00007890-200109150-00031
7. Assadiasl S, Toosi MN, Mohebbi B, Ansaripour B, Soleimanifar N, Sadr M, et al. Th17/Treg cell balance in stable liver transplant recipients. Transpl Immunol. 2022;71:101540. doi: 10.1016/j.trim.2022.101540.
8. Schroder K, Hertzog PJ, Ravasi T, Hume DA. Interferon-γ: An overview of signals, mechanisms and functions. J Leukoc Biol. 2004;75(2):163-89. doi: 10.1189/jlb.0603252.
9. Millán O, Rafael-Valdivia L, San Segundo D, Boix F, Castro-Panete MJ, López-Hoyos M, et al. Should IFN-γ, IL-17 and IL-2 be considered predictive biomarkers of acute rejection in liver and kidney transplant? Results of a multicentric study. Clin Immunol. 2014;154(2):141-54. doi: 10.1016/j.clim.2014.07.007.
10. Lord R, Goto S, Pan T-L, Chiang K-C, Chen C-L, Sunagawa M. Peak Protein Expression of Il-2 And Ifn-Γ Correlate with The Peak Rejection Episode In a Spontaneously Tolerant Model of Rat Liver Transplantation. Cytokine. 2001;13(3):155-61. doi: 10.1006/cyto.2000.0815.
11. Niu J, Yue W, Song Y, Zhang Y, Qi X, Wang Z, et al. Prevention of acute liver allograft rejection by IL-10-engineered mesenchymal stem cells. Clin Exp Immunol. 2014;176(3):473-84. doi: 10.1111/cei.12283.
12. Wan R, Tang L, Shan R, Zeng L, Chen H, Gao L. Humoral immunity-mediated chronic rejection in liver transplantation is associated with predominant IL-10 expression. Front Biosci (Elite Ed). 2012;4:2121-30. doi: 10.2741/529.
13. Mariotti J, Foley J, Ryan K, Buxhoeveden N, Kapoor V, Amarnath S, et al. Graft rejection as a Th1-type process amenable to regulation by donor Th2-type cells through an interleukin-4/STAT6 pathway. Blood .2008;112(12):4765-75. doi: 10.1182/blood-2008-05-154278
14. Heeger PS. T-cell allorecognition and transplant rejection: a summary and update. Am J Transplant. 2003;3(5):525-33. doi: 10.1034/j.1600-6143.2003.00123.x.
15. Amarnath S, Chen H, Foley JE, Costanzo CM, Sennesh JD, Solomon MA, et al. Host-based Th2 cell therapy for prolongation of cardiac allograft viability. PLoS One. 2011;6(4):e18885. doi: 10.1371/journal.pone.0018885.
16. Liu Z, Fan H, Jiang S. CD4+ T‐cell subsets in transplantation. Immunol rev. Immunol Rev. 2013 Mar;252(1):183-91. doi: 10.1111/imr.12038.
17. Ganschow R, Broering DC, Nolkemper D, Albani J, Kemper MJ, Rogiers X, et al. Th2 cytokine profile in infants predisposes to improved graft acceptance after liver transplantation. Transplantation. 2001 Sep 15;72(5):929-34. doi: 10.1097/00007890-200109150-00031.
18. Chen Y, Chen J, Liu Z, Liang S, Luan X, Long F, et al. Relationship Between TH1/TH2 Cytokines and Immune Tolerance in Liver Transplantation in Rats. Transplant Proc. 2008;40(8):2691-5. doi: 10.1016/j.transproceed.2008.08.014.
19. Wang Yl, Tang Zq, Gao W, Jiang Y, Zhang Xh, Peng L. Influence of Th1, Th2, and Th3 cytokines during the early phase after liver transplantation. Transplant Proc. 2003;35(8):3024-5. doi: 10.1016/j.transproceed.2003.10.007.
20. Park CS, Moon HY, Han S, Chon JY, Chae MS, Hong SH, et al. Ischemic time of graft liver forces Th1-to-Th2 activity toward Th1 activity in patients who underwent living donor liver transplantation. Eur Cytokine Netw. 2019;30(1):23-8. doi: 10.1684/ecn.2019.0422.
21. Demirkiran A, Kok A, Kwekkeboom J, Metselaar HJ, Tilanus HW, van der Laan LJ. Decrease of CD4+CD25+ T cells in peripheral blood after liver transplantation: association with immunosuppression. Transplant Proc. 2005;37(2):1194-6. doi: 10.1016/j.transproceed.2004.12.095.
22. Li S, Yu J, Guo C, Jie Y, Pan Z. The Balance of Th1/Th2 and LAP+Tregs/Th17 Cells Is Crucial for Graft Survival in Allogeneic Corneal Transplantation. J Ophthalmol. 2018;2018:5404989. doi: 10.1155/2018/5404989.
23. Assadiasl S, Mooney N, Nicknam MH. Cytokines in Liver Transplantation. Cytokine. 2021;148:155705. doi: 10.1016/j.cyto.2021.155705.
24. Saraiva M, O'Garra A. The regulation of IL-10 production by immune cells. Nat Rev Immunol. 2010;10(3):170-81. doi: 10.1038/nri2711.
25. Hassan L, Bueno P, Ferrón-Celma I, Ramia J, Garrote D, Muffak K, et al. Early postoperative response of cytokines in liver transplant recipients. Transplant Proc. 2006 Oct;38(8):2488-91. doi: 10.1016/j.transproceed.2006.08.054.
26. König R, Kolte A, Ahlers O, Oswald M, Krauss V, Roell D, et al. Use of IFNγ/IL10 Ratio for Stratification of Hydrocortisone Therapy in Patients With Septic Shock. Front Immunol. 2021 Mar 9:12:607217. doi: 10.3389/fimmu.2021.607217.
27. Ala Y, Pasha MK, Rao RN, Komaravalli PL, Jahan P. Association of IFN-γ : IL-10 Cytokine Ratio with Nonsegmental Vitiligo Pathogenesis. Autoimmune Dis. 2015;2015:423490-. doi: 10.1155/2015/423490.
28. Giunta EF, Barra G, De Falco V, Argenziano G, Napolitano S, Vitale P, et al. Baseline IFN-γ and IL-10 expression in PBMCs could predict response to PD-1 checkpoint inhibitors in advanced melanoma patients. Sci Rep. 2020;10(1):17626. doi: 10.1038/s41598-020-72711-2.
29. van den Boogaardt DE, van Miert PP, de Vaal YJ, de Fijter JW, Claas FH, Roelen DL. The ratio of interferon-gamma and interleukin-10 producing donor-specific cells as an in vitro monitoring tool for renal transplant patients. Transplantation. 2006;82(6):844-8. doi: 10.1097/01.tp.0000229448.64363.18.
30. Attia F, Kheder E, Khalil K, Tawfik G, Omar W, Anani M. Assessment of Interferon Gamma and Interleukin-10 m-RNA Expression in Peripheral Blood From Renal Transplanted Patients with Chronic Allograft Nephropathy by Real Time Polymerase Chain Reaction. Austin J Nephrol Hypertens. 2015;2(5):1050. id1050 (2015)
31. Chen Y, Chen J, Liu Z, Liang S, Luan X, Long F, et al. Relationship between TH1/TH2 cytokines and immune tolerance in liver transplantation in rats. Transplant Proc. 2008 Oct;40(8):2691-5. doi: 10.1016/j.transproceed.2008.08.014.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Assadiasl S, Nassiri Toosi M, Soleimanifar N, Mohebbi B, Ansaripour B, Sadr M, et al . T helper1/T helper2 and Interferon-γ/Interleukin-10 Ratios in Liver Transplant Rejection. Journal of Basic Research in Medical Sciences 2024; 11 (1) :45-57
URL: http://jbrms.medilam.ac.ir/article-1-782-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 11, Issue 1 (1-2024) Back to browse issues page
مجله ی تحقیقات پایه در علوم پزشکی Journal of Basic Research in Medical Sciences
Persian site map - English site map - Created in 0.16 seconds with 41 queries by YEKTAWEB 4654